Trimethoprim ELISA Kit
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Part No | Product Name | Applicable Samples |
|---|---|---|
9B2019-01 | Trimethoprim ELISA kit
| Livestock/Poultry Muscle/Organs/Eggs |
Principle
This kit is based on indirect-competitive ELISA technology. The microtiter wells are coated with coupling antigen. Trimethoprim residue in the sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of enzyme labeled anti-antibody, TMB substrate is used to show the color. Absorbance of the sample is negatively related to the Trimethoprim reside in it, after comparing with the Standard Curve, multiplied by the dilution multiple, Trimethoprim residue quantity in the sample can be calculated.
Detection Limit (LOD)
0.02ppb
Kit Components
- Microtiter plate with 96 wells precoated with antigen
- Standard solutions concentrate (6×1ml/bottle)
0ppb, 0.02ppb, 0.06ppb,0.18ppb, 0.54ppb, 1.62 ppb - Spiking standard control: (1ml/bottle) 1ppm
- Enzyme conjugate concentrate 1ml
- Trimethoprim Antibody solution 10ml
- Solution A 7ml
- Solution B 7ml
- Stop solution 7ml
- 20×concentrated wash solution 40ml
- 2×Extraction solution 50ml
Product Information
Detectable Samples, Detection Limit and Recovery Rate
Sample Name | Detection Limit | Recovery Rate |
|---|---|---|
Animal Tissue
| 3 .2 ppb | 100 ± 30% |
Eggs | 3 ppb | 100 ± 30% |
Test Specificity
Chinese Name | English Name | Cross-Reactivity Rate |
|---|---|---|
四環黴素 | Tetracycline | 100% |
氯四環黴素 | Chlortetracycline | 140% |
鏈黴素 | Streptomycin | 58% |
脫氧羥四環黴素 | Doxycycline | 76% |
米諾四環素 | Minocycline | 170% |
去甲基氯四環素 | Demethylchlortetracycline | 86% |
吡甲四環素 | Rolitetracycline | 78% |
other | <1% |
Product Specification
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