Tetracyclines ELISA Kit
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Part No | Product Name | Applicable Samples |
|---|---|---|
9B2037-01 | Tetracyclines ELISA Kit
| Livestock/Poultry Muscle/Organs/Eggs
|
Principle
This kit is based on indirect-competitive ELISA technology. The microtiter wells are coated with coupling antigen. Tetracyclines residue in the sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of enzyme labeled anti-antibody, TMB substrate is used to show the color. Absorbance of the sample is negatively related to the Tetracyclines reside in it, after comparing with the Standard Curve, multiplied by the dilution multiple, Tetracyclines residue quantity in the sample can be calculated.
Detection Limit (LOD)
0.2ppb
Kit Components
- Microtiter plate with 96 wells precoated with antigen
- Standard solutions concentrate (5×1ml/bottle)
0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb - Spiking standard control: (1ml/bottle) 1ppm
- Enzyme conjugate concentrate 1ml
- Tetracyclines Antibody solution 10ml
- Solution A 7ml
- Solution B 7ml
- Stop solution 7ml
- 20×concentrated wash solution 40ml
- 2×Extraction solution 50ml
Product Information
Detectable Samples, Detection Limits and Recovery Rates
Sample Type | Detection Limit | Recovery Rate |
|---|---|---|
Animal Tissue
| 0.02 ppb | 80 ± 20% |
Egg | 0.02 ppb | 80 ± 20% |
Test Specificity
Chinese Name | English Name | Cross-Reactivity Rate |
|---|---|---|
三甲氧芐氨嘧啶 | Trimethoprim | 100% |
二甲氧芐氨嘧啶 | Diaveridine | <1% |
Product Specification
Pictures are for reference only, please refer to the actual item.